9ICC
DNA POLYMERASE BETA (E.C.2.7.7.7)/DNA COMPLEX + 2'-DEOXYADENOSINE-5'-TRIPHOSPHATE, SOAKED IN THE PRESENCE OF DATP AND CRCL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1995-03-29 |
Detector | SDMS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 179.300, 57.603, 48.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.157 * |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 8icb |
RMSD bond length | 0.019 |
RMSD bond angle | 19.000 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT (V. 5-D) |
Refinement software | TNT (5-D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.065 * | 0.179 * |
Number of reflections | 8833 | |
Completeness [%] | 92.0 | 92 |
Redundancy | 2 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | 298 * | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A COCRYSTAL OF HUMAN DNA POLYMERASE BETA COMPLEXED WITH 7 BASE PAIRS OF DNA (SEE ENTRY 9ICJ AND REFERENCE 1) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 24 HOURS: PEG 3350, 16% MES, 100 MILLIMOLAR, PH 6.5 NACL, 150 MILLIMOLAR DATP, 1 MILLIMOLAR CRCL3, 5 MILLIMOLAR SEE REFERENCE 2 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 15-17 (%) | |
2 | 1 | reservoir | citrate | 0.1 (M) | |
3 | 1 | reservoir | 50 (mM) |