9IC7
Cryo-EM structure of alpha-synuclein fibrils formed in artificial cerebrospinal fluid (aCSF)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.9 |
| Software | RELION,PHENIX,EPU,CTFFIND,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
