9IBR

Crystal structure of HNF4 alpha LBD complexed with GRIP-1 peptide and ESY13

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-02
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.8731
Spacegroup name	P 42 21 2
Unit cell lengths	87.769, 87.769, 62.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.120 - 2.780
R-factor	0.2923
Rwork	0.290
R-free	0.35460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.522
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.120	3.031
High resolution limit [Å]	2.130	2.777
Number of reflections	4630	231
<I/σ(I)>	6.1
Completeness [%]	92.5
Redundancy	2.69
CC(1/2)	0.997	0.103

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	16% PEG3350 8% ethylene glycol 0.1M bis-tris-propane pH 8.5 -