9IBO

VC0430/VcGluP bound to glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.03321
Spacegroup name	H 3 2
Unit cell lengths	128.827, 128.827, 178.484
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.300 - 2.080
R-factor	0.1675
Rwork	0.166
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.625
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	2.130
High resolution limit [Å]	2.080	2.080
Number of reflections	66422	4730
<I/σ(I)>	9.99
Completeness [%]	99.9
Redundancy	5.2
CC(1/2)	0.998	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.1 M malic acid (pH 6.5) 13% glycerol