9IAE
Structure of a Chimeric Protein Composed of the SNX5 PX Domain and the N-Terminal Region of NCOA7-AS, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-22 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.130, 63.130, 101.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.130 - 2.300 |
| R-factor | 0.2053 |
| Rwork | 0.201 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.193 | 1.660 |
| Number of reflections | 10729 | 1269 |
| <I/σ(I)> | 16.02 | 2.2 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 19.6 | 20.14 |
| CC(1/2) | 0.990 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Calcium acetate hydrate 0.1 M Sodium Cacodylate pH 6.5 18% PEG 8000 |
