9IAD

Chroococcidiopsis thermalis Argonaute (CtAgo) bound to the 3' end of a guide DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003
Spacegroup name	P 1 21 1
Unit cell lengths	74.901, 50.590, 98.572
Unit cell angles	90.00, 102.75, 90.00

Refinement procedure

Resolution	48.070 - 2.400
R-factor	0.2236
Rwork	0.222
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.758
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.070	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.085	1.467
Number of reflections	28341	2692
<I/σ(I)>	12.06	0.96
Completeness [%]	99.0
Redundancy	4
CC(1/2)	1.000	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein solution: -3.74 mg/ml CtAgo -500 mM KCl -20 mM HEPES-KOH pH7.5 -1 mM DTT Reservoir solution: -0.05 M Ammonium Formate -8% PEG3350