9IAB
Structure of the Argonaute-associated Cas4 family protein 1 (ACE1) from Chroococcidiopsis thermalis (CtACE1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.910, 44.950, 74.060 |
| Unit cell angles | 100.17, 92.22, 105.87 |
Refinement procedure
| Resolution | 43.020 - 1.570 |
| R-factor | 0.1863 |
| Rwork | 0.186 |
| R-free | 0.19920 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.020 | 1.630 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmeas | 0.034 | 0.559 |
| Number of reflections | 144407 | 7241 |
| <I/σ(I)> | 31.65 | |
| Completeness [%] | 99.3 | 94.57 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.999 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein solution: -7.5 mg/ml CtACE1 -10 mM HEPES-KOH pH7.5 -250 mM KCl -1 mM DTT Reservoir solution: -0.1 M MES pH 6-7 -0.2 M (NH4)2SO4 -20-25% PEG MME 5K |
