9I9M

Crystal structure of chimeric UFC1, TAK MotiF replaced with HPN motif of other E2 proteins

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-26
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.87313
Spacegroup name	P 41 21 2
Unit cell lengths	47.163, 47.163, 144.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.839 - 1.543
Rwork	0.190
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.583
Data reduction software	autoPROC
Data scaling software	autoPROC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.840	1.598
High resolution limit [Å]	1.543	1.543
Rmeas	0.039	0.957
Number of reflections	24971	2414
<I/σ(I)>	12.8	0.81
Completeness [%]	99.9	98.65
Redundancy	2	2
CC(1/2)	0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1M sodium citrate tribasic dihydrate pH 5.5, 20% w/v 2-propanol, 20% w/v PEG 4000