9I9F
Crystal structure of apoform human eIF4A1 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 |
| Unit cell lengths | 84.970, 84.970, 66.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.003 - 2.731 |
| Rwork | 0.229 |
| R-free | 0.29370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.438 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.490 | 2.780 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmeas | 0.189 | 1.375 |
| Number of reflections | 14242 | 730 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.994 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 18.5% (w/v) PEG 8000, 0.2M sodium acetate trihydrate, 0.1M MES, pH 6.0 |
