9I6M

Hen egg-white lysozyme structure at 65% relative humidity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	297
Detector technology	PIXEL
Collection date	2024-06-15
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.976
Spacegroup name	P 43 21 2
Unit cell lengths	79.000, 79.000, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.860 - 1.700
R-factor	0.163
Rwork	0.161
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.521
Data reduction software	CrystFEL (0.10.1)
Data scaling software	CrystFEL (0.10.1)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.860	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	13878	1341
<I/σ(I)>	7.62	2.5
Completeness [%]	99.7	99.8
Redundancy	234.4	138.1
CC(1/2)	0.983	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	3	297	20% NaCl, 6% PEG 6000, 0.1M sodium acetate pH 3.0