9I6J
Hen egg-white lysozyme structure embedded in LCP medium at 95% relative humidity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2024-06-15 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.000, 79.000, 38.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.860 - 1.700 |
| R-factor | 0.1616 |
| Rwork | 0.160 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.983 |
| Data reduction software | CrystFEL (0.10.1) |
| Data scaling software | CrystFEL (0.10.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.860 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 13757 | 1344 |
| <I/σ(I)> | 5.22 | 1.58 |
| Completeness [%] | 100.0 | |
| Redundancy | 124.9 | |
| CC(1/2) | 0.964 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 3 | 297 | 20% NaCl, 6% PEG 6000, 0.1M sodium acetate pH 3.0 |
