9I6H
Room temperature structure of KR2 rhodopsin in pentameric form at 95% relative humidity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2023-07-11 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.100, 240.900, 138.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.250 - 3.000 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.531 |
| Data reduction software | CrystFEL (0.10.1) |
| Data scaling software | CrystFEL (0.10.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.250 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 45854 | 4499 |
| <I/σ(I)> | 2.65 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 89 | 61.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 297 | 3.2M sodium malonate pH 8.0 |
