9I68
Crystal structure of human Cdc20 bound to synthetic D-box peptide D21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-24 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.491, 87.550, 48.573 |
| Unit cell angles | 90.00, 109.71, 90.00 |
Refinement procedure
| Resolution | 45.730 - 1.505 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.730 | 1.666 |
| High resolution limit [Å] | 1.505 | 1.509 |
| Rmerge | 0.050 | 0.598 |
| Rmeas | 0.056 | 0.688 |
| Rpim | 0.024 | 0.333 |
| Number of reflections | 32820 | 1641 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 74.7 | |
| Redundancy | 5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 10 % MPD, 12-14 % PEG 6000, 0.1 M MES pH 6.5 |
