9I63
Synthetic Human Saposin D glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918007 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.440, 65.680, 42.470 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 36.799 - 1.650 |
| Rwork | 0.161 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.013 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.091 | 0.423 |
| Rmeas | 0.105 | 0.569 |
| Number of reflections | 23455 | 659 |
| <I/σ(I)> | 13.73 | 2.3 |
| Completeness [%] | 96.0 | 78.8 |
| Redundancy | 3.12 | 2.04 |
| CC(1/2) | 0.987 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4 M (NH4)2SO4, 0.2 M NH4OAc, 20 mM Tris pH 7.5 |
