9I56
CRYSTAL STRUCTURE OF HUMAN MONOACYLGLYCEROL LIPASE WITH COMPOUND 23
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-08 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.885603 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.046, 127.382, 62.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.690 - 1.490 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.980 | 1.590 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.100 | 0.980 |
| Number of reflections | 58359 | 10179 |
| <I/σ(I)> | 7.77 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.05 | |
| CC(1/2) | 0.998 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.5, 9% PEG MME5K, 12% isopropanol |
