9I55

Mouse phosphomannomutase 2 in complex with the activator glucose-1,6-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	81.285, 99.138, 213.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.670 - 2.750
R-factor	0.2084
Rwork	0.205
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.685
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	213.470	2.850
High resolution limit [Å]	2.750	2.750
Rpim	0.097	0.883
Number of reflections	45273	4408
<I/σ(I)>	6.6	1
Completeness [%]	98.0	100
Redundancy	6.6	6.6
CC(1/2)	0.993	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	Drops of 2 ul of protein and 2 mM glucose-1,6-bisphosphate (Sigma) and 2 ul of reservoir solution. Best crystals appeared after 2-7 days in 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, 20-22% PEG 8000. Crystals were cryo-protected by briefly soaking in mother liquor with increasing concentrations of PEG up to 30% and 5% glycerol.