9I4E
Structure of anti-EV71 human monoclonal antibody 34-1-6D Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.164, 55.981, 75.399 |
| Unit cell angles | 86.69, 86.39, 67.06 |
Refinement procedure
| Resolution | 75.200 - 1.630 |
| R-factor | 0.16077 |
| Rwork | 0.158 |
| R-free | 0.20884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.373 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.200 | 1.672 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 98557 | 4808 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 30% (w/v) PEG 3000, 0.1 M CHES, pH 9.6 |
