9I4D

Structure of anti-EV71 human monoclonal antibody 17-2-2B Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	72.480, 71.660, 93.060
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	49.980 - 2.560
R-factor	0.1965
Rwork	0.194
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.582
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.980	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	29400	1109
<I/σ(I)>	9.7
Completeness [%]	98.6
Redundancy	11.9
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	30% (w/v) polyethylene glycol monomethyl ether 2000, 0.1 M sodium acetate, pH 4.6, 0.2 M ammonium sulphate