9I4C

Structure of anti-EV71 human monoclonal antibody 17-1-12A Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9282
Spacegroup name	P 6 2 2
Unit cell lengths	152.750, 152.750, 120.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.370 - 2.830
R-factor	0.2248
Rwork	0.223
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.575
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.370	2.980
High resolution limit [Å]	2.830	2.830
Number of reflections	20091	994
<I/σ(I)>	7.6
Completeness [%]	98.7
Redundancy	9.8
CC(1/2)	0.980	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.5 M lithium sulphate mon, 0.1 M HEPES-Na, pH 7.5