9I45

Structure of anti-EV71 human monoclonal antibody 16-3-10B Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9282
Spacegroup name	P 1 21 1
Unit cell lengths	67.803, 50.059, 74.471
Unit cell angles	90.00, 116.52, 90.00

Refinement procedure

Resolution	60.670 - 2.120
R-factor	0.2055
Rwork	0.204
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.494
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.670	2.200
High resolution limit [Å]	2.120	2.120
Number of reflections	25701	1197
<I/σ(I)>	9
Completeness [%]	99.7
Redundancy	6.3
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	30% (w/v) PEG 6000, 0.1 M HEPES, pH 7.0