9I43
Structure of anti-EV71 human monoclonal antibody 16-2-9D Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.743, 80.316, 57.489 |
| Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
| Resolution | 57.341 - 1.180 |
| Rwork | 0.145 |
| R-free | 0.17580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.780 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.341 | 1.211 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Number of reflections | 141520 | 7009 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 25% (w/v) PEG 3350, 0.1 M citrate, pH 3.5 |
