9I41

Structure of anti-EV71 human monoclonal antibody 16-3-3C Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	77.826, 156.587, 78.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.416 - 1.320
Rwork	0.143
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.727
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.416	1.354
High resolution limit [Å]	1.320	1.320
Number of reflections	112322	5448
<I/σ(I)>	18.9
Completeness [%]	99.9
Redundancy	69.4
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	25% (w/v) PEG 3350, 0.1 M bis-Tris, pH 5.5