9I3Z

Structure of anti-EV71 human monoclonal antibody 16-2-12D Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9282
Spacegroup name	C 2 2 2
Unit cell lengths	130.210, 265.334, 81.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.710 - 3.060
R-factor	0.2118
Rwork	0.209
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.648
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.710	3.160
High resolution limit [Å]	3.060	3.060
Number of reflections	27241	1299
<I/σ(I)>	8.1
Completeness [%]	99.9
Redundancy	26
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (w/v) PEG 3350, 0.2 M sodium di-hydrogen phosphate