9I3X

Structure of anti-EV71 human monoclonal antibody 16-2-11B Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9282
Spacegroup name	P 1 21 1
Unit cell lengths	75.319, 83.945, 84.955
Unit cell angles	90.00, 103.50, 90.00

Refinement procedure

Resolution	82.610 - 2.570
R-factor	0.211
Rwork	0.209
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.687
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.610	2.650
High resolution limit [Å]	2.570	2.570
Number of reflections	32644	1595
<I/σ(I)>	14.4
Completeness [%]	99.0
Redundancy	6.7
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (w/v) PEG 3350, 0.2 M sodium formate