9I3W

Structure of anti-EV71 human monoclonal antibody 16-2-8C Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9282
Spacegroup name	P 1 21 1
Unit cell lengths	47.013, 80.394, 57.572
Unit cell angles	90.00, 93.83, 90.00

Refinement procedure

Resolution	46.908 - 1.140
Rwork	0.166
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.635
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.908	1.169
High resolution limit [Å]	1.140	1.140
Number of reflections	153940	7168
<I/σ(I)>	11.5
Completeness [%]	99.1
Redundancy	6
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	25% (w/v) PEG 3350, 0.1 M Bis-Tris, pH 6.5, protein concentration: 26.4mg/ml