9I2S

Velvet domain of the transcriptional regulator VeA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8000
Spacegroup name	P 21 21 2
Unit cell lengths	106.040, 156.080, 52.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.210 - 2.400
R-factor	0.2209
Rwork	0.219
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.701
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.210	50.000	2.500
High resolution limit [Å]	2.400	17.000	2.400
Rmerge	0.041	0.007	1.821
Rmeas	0.047	0.009	2.078
Number of reflections	34384	108	3879
<I/σ(I)>	22.93
Completeness [%]	98.0
Redundancy	4.18
CC(1/2)	1.000	1.000	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% PEG 3350 200 mM (NH4)2SO4 100 mM BisTris/HCl