9I2J
Branching Sucrase BRS-B in complex with Sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97929 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 255.133, 255.133, 344.915 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.262 - 3.100 |
| Rwork | 0.193 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.226 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.630 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.734 | |
| Number of reflections | 76886 | 37306 |
| <I/σ(I)> | 4.4 | 1 |
| Completeness [%] | 98.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1M SPG Buffer pH 7, 20% PEG 1500 |
