9I25
WxLIP from Enterococcus faecium locus A bound to long WxL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2023-07-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 61 |
| Unit cell lengths | 105.147, 105.147, 67.172 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.000 - 1.911 |
| Rwork | 0.217 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.312 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 5.180 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.251 | 5.740 |
| Rmeas | 0.257 | 5.878 |
| Rpim | 0.056 | 1.259 |
| Number of reflections | 32902 | 1646 |
| <I/σ(I)> | 8.1 | 0.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.1 | 21.7 |
| CC(1/2) | 0.998 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | sodium HEPES, PEG12000 |
