9I23

Structure of Human helicase RecQ1- 795-2T G-quadruplex - ADP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978470
Spacegroup name	P 41 21 2
Unit cell lengths	155.659, 155.659, 129.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.830 - 3.690
R-factor	0.2444
Rwork	0.244
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.807
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.830	3.760
High resolution limit [Å]	3.690	3.690
Rmerge	0.140	1.628
Number of reflections	17359	862
<I/σ(I)>	16.1	2.2
Completeness [%]	98.4	100
Redundancy	13.6	14.3
CC(1/2)	0.998	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Malonate 0.1M pH 7 PEG 3350 12%