9I1T

CARM1 in complex with arg-MMA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	75.272, 99.167, 208.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 2.390
R-factor	0.22
Rwork	0.219
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.512
Data reduction software	XDS (0.7.8)
Data scaling software	Aimless
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	2.450
High resolution limit [Å]	2.390	2.390
Rmerge	0.185	2.458
Rmeas	0.193	2.726
Rpim	0.052	0.759
Number of reflections	62374	4275
<I/σ(I)>	10	0.9
Completeness [%]	99.2	93.7
Redundancy	13.4	12.4
CC(1/2)	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	9% PEG3350, 100mM Tris HCl pH7.5, 0.2M ammonium sulfate