9I1P

Structure of RecQL- Myc G-quadruplex - ADP complex from Bos taurus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978470
Spacegroup name	C 1 2 1
Unit cell lengths	125.437, 126.417, 198.198
Unit cell angles	90.00, 100.90, 90.00

Refinement procedure

Resolution	76.550 - 3.200
R-factor	0.2464
Rwork	0.244
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.550	3.490
High resolution limit [Å]	3.200	3.200
Rmerge	0.193	1.196
Number of reflections	37700	1186
<I/σ(I)>	8	1.6
Completeness [%]	86.9	34.3
Redundancy	6.6	6
CC(1/2)	0.994	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Sodium Nitrate 0.3M Sodium Phosphate dibasique 0.3M Ammonium Sulfate 0.3M 0.1M Tris Bicine ph 8.5 12% Ethylene Glycol 6% PEG 8000