9I16
Structure of RecQL-dsDNA-ADP-AlF4 complex from Bos taurus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978510 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.559, 112.082, 181.788 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 29.890 - 2.820 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.890 | 3.030 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.219 | 1.389 |
| Number of reflections | 78828 | 3941 |
| <I/σ(I)> | 5.3 | 1.5 |
| Completeness [%] | 94.6 | 67.7 |
| Redundancy | 5.3 | 6.2 |
| CC(1/2) | 0.977 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | Tacsimate 5% HEPES 0.1M PEG MME 5000 10% |
