9I0S

Structure of RecQL-ADP complex from Bos taurus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	55.153, 119.450, 201.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.480 - 2.500
R-factor	0.2412
Rwork	0.240
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.480	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.214	3.118
Number of reflections	47036	2275
<I/σ(I)>	8.2	1
Completeness [%]	100.0	100
Redundancy	11.1	11.5
CC(1/2)	0.994	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	Tris-HCl 20mM KCl 100mM DTT 1mM