9I09
The Paulinella chromatophore transit peptide part2 (crTPpart2) of RnaH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976260 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.164, 81.164, 63.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.150 - 1.990 |
| R-factor | 0.1932 |
| Rwork | 0.190 |
| R-free | 0.22410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.941 |
| Data reduction software | autoXDS (1.1.7) |
| Data scaling software | XSCALE (1.1.7) |
| Phasing software | PHASER (3.4.5) |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.150 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 16511 | 1373 |
| <I/σ(I)> | 21.26 | 2.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | 23% PEG 3350 in 0.1 M HEPES pH 8.5 |
