9HYX
AlfB fucosidase in complex with Fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97928 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.299, 309.675, 173.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.290 - 2.300 |
| R-factor | 0.17439 |
| Rwork | 0.173 |
| R-free | 0.19651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.063 | 0.438 |
| Number of reflections | 200086 | 9847 |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 3% PEG 8000, 25% MPD, 7% Taximate pH 5 |
