9HYR
Crystal structure of a peptidase N4 domain of D29-LysA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.170, 66.976, 82.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.250 - 3.200 |
| R-factor | 0.2491 |
| Rwork | 0.246 |
| R-free | 0.31440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.970 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.173 | 0.376 |
| Number of reflections | 6267 | 1110 |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 4.4 | 4.5 |
| CC(1/2) | 0.934 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES pH 7.5, 1.8M NaCl |
