9HYQ
BT984 a GH139 rhamnogalacturonan II exo-a-1,2-(2-Omethyl)-fucosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.201, 138.322, 195.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.478 - 2.700 |
Rwork | 0.218 |
R-free | 0.25570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.478 | 2.760 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 75053 | 4450 |
<I/σ(I)> | 10.4 | 1.3 |
Completeness [%] | 98.9 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20 % PEG 3000 0.1 M Tris pH8.5 10 mg/ml |