9HYE
Structure of the Mus musclus Langerin carbohydrate recognition domain with depleted Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.917, 80.706, 52.179 |
| Unit cell angles | 90.00, 105.25, 90.00 |
Refinement procedure
| Resolution | 40.350 - 1.640 |
| R-factor | 0.1566 |
| Rwork | 0.154 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmeas | 0.161 | 2.109 |
| Number of reflections | 34064 | 5380 |
| <I/σ(I)> | 8.1 | 1 |
| Completeness [%] | 98.9 | 96.6 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.994 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 21% (w/v) polyethylene glycol 3350 and 200 mM KCl |
