9HY1

sc-4E (scFv derived from the mAb 4E1 against CD93) crystallized at pH 7.5 (dimeric state from gel filtration)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-10
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.95374
Spacegroup name	P 32 2 1
Unit cell lengths	64.369, 64.369, 159.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.750 - 1.810
R-factor	0.17047
Rwork	0.169
R-free	0.19633
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.159
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.940	1.850
High resolution limit [Å]	1.810	1.810
Rmerge	0.047	1.755
Rmeas	0.048	1.802
Rpim	0.011	0.407
Number of reflections	35952	2092
<I/σ(I)>	28	2
Completeness [%]	100.0	100
Redundancy	20.2	19.4
CC(1/2)	1.000	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	3.8 M NaCl, 0.1 M HEPES pH 7.5