9HV1
Crystal structure of tri-specific FMDV mAb-17 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 61 |
| Unit cell lengths | 136.092, 136.092, 79.673 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.550 - 1.960 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.798 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.138 | |
| Rpim | 0.031 | |
| Number of reflections | 60338 | 3048 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 21.1 | |
| CC(1/2) | 0.999 | 0.384 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M bicine pH 8.5, 15 % Polyethylene glycol (PEG) 1,500 |
