9HU9

Glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system. Complex with UDP-GlcNAc.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	76.639, 121.392, 174.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.700 - 2.400
R-factor	0.1836
Rwork	0.181
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.978
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.700	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.099	0.902
Number of reflections	64276	6327
<I/σ(I)>	5.62	0.58
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.990	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	289	ammonium hydrogen citrate, PEG 3350