9HU7

Mutant M298C6a of the small laccase (SLAC) from Streptomyces coelicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-06
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 43 3 2
Unit cell lengths	178.755, 178.755, 178.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.590 - 3.510
R-factor	0.2055
Rwork	0.205
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (2.0rc1-5599)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.200	3.590
High resolution limit [Å]	3.490	3.490
Rmerge	0.391	1.370
Rmeas	0.424	1.467
Number of reflections	12753	623
<I/σ(I)>	5.4	1.7
Completeness [%]	99.8	62.7
Redundancy	6.6
CC(1/2)	0.961	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	18.2% Jeffamine-M600 and 17.5% DMSO