9HTS
human KHNYN KH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.833, 121.797, 44.547 |
| Unit cell angles | 90.00, 108.55, 90.00 |
Refinement procedure
| Resolution | 60.899 - 2.198 |
| Rwork | 0.196 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.899 | 2.260 |
| High resolution limit [Å] | 2.198 | 2.200 |
| Number of reflections | 10852 | 183 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 73.3 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.995 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Tris, 250 mM NaCl, 35 % PEG 3000, pH 7.0 |
