9HTP

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with glutarimide based compound 2p

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-21
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 21
Unit cell lengths	55.831, 59.800, 86.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 2.350
R-factor	0.24044
Rwork	0.238
R-free	0.28066
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.406
Data scaling software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.350	2.350
Rmeas	0.132
Number of reflections	23376	3748
<I/σ(I)>	9.51	1.6
Completeness [%]	100.0	99.9
Redundancy	6.9	7.1
CC(1/2)	0.997	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3mM thalidomide