9HTN
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with glutarimide based compound 2s
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-20 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.470, 60.042, 88.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 1.920 |
| R-factor | 0.19272 |
| Rwork | 0.191 |
| R-free | 0.23532 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.740 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.690 | 2.030 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 22809 | 2900 |
| <I/σ(I)> | 9.3 | 1.22 |
| Completeness [%] | 96.4 | 77.9 |
| Redundancy | 12.56 | 13.33 |
| CC(1/2) | 0.996 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3mM thalidomide |
