9HT6

Crystal structure of Tetraspanin CD82 Large Extracellular Loop (LEL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.999
Spacegroup name	P 21 21 21
Unit cell lengths	51.420, 62.670, 97.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.400
R-factor	0.2288
Rwork	0.226
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.422
Data reduction software	XDS (20240712)
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.810	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	12818	1218
<I/σ(I)>	24.79	1.54
Completeness [%]	99.6	97.13
Redundancy	10.52
CC(1/2)	1.000	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	1.0 M Ammonium phosphate dibasic, 0.1 M Sodium acetate