9HS2

Crystal structure of the Escherichia coli nucleosidase PpnN (ppGpp form)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.54180
Spacegroup name	P 43 21 2
Unit cell lengths	155.115, 155.115, 226.088
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.110 - 3.400
R-factor	0.2223
Rwork	0.219
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gfm
RMSD bond length	0.011
RMSD bond angle	1.469
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.110	3.520
High resolution limit [Å]	3.400	3.400
Rmeas	0.090	1.640
Number of reflections	39210	3779
<I/σ(I)>	11.11
Completeness [%]	99.3	95.6
Redundancy	2
CC(1/2)	0.990	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	6.6 mg/ml 0.1 M HEPES pH 7.5 4% w/v PEG8000