9HRS
ZnT1 CTD regulation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.595, 71.394, 67.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.800 - 1.920 |
| R-factor | 0.2279 |
| Rwork | 0.225 |
| R-free | 0.28015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.837 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.800 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 5.470 | |
| Number of reflections | 13179 | 868 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13 | |
| CC(1/2) | 1.000 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M tri-sodium citrate pH 5.6, 0.2M potassium sodium tartrate, 2M ammonium sulfate |
