9HRO

Solution NMR structure of the synthetic tobramycin riboswitch in complex with tobramycin

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE NEO		600
2	Bruker	AVANCE III HD		700
3	Bruker	AVANCE III HD		800
4	Bruker	AVANCE NEO		900
5	Bruker	AVANCE III		950
6	Bruker	AVANCE II		600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-1H NOESY
13	1	2	2D long-range 1H-15N HSQC
14	1	2	3D 1H-15N NOESY amino
15	1	3	2D H5(C5C4N4)H4
16	1	5	2D 1H-13C HSQC aliphatic
17	1	7	2D 1H-13C HSQC aromatic
18	1	5	2D H1/H8-C5 HMBC
19	1	5	2D H(N)CO
2	1	2	2D 1H-15N HSQC imino
20	2	6	2D H(C)N aromatic
21	2	4	2D H(C)N aromatic
22	2	6	2D H(C)N aliphatic
23	2	4	2D H(C)N aliphatic
24	2	6	3D 1H-13C NOESY-HSQC aro
25	2	6	3D 1H-13C NOESY-HSQC ali
26	2	4	3D 1H-13C NOESY-HSQC ali
27	2	6	3D HCCH-COSY
28	2	4	3D HCCH-COSY
29	2	6	3D HCCH-TOCSY
3	1	2	2D 1H-15N HSQC amino
30	2	4	3D HCCH-TOCSY
31	1	7	2D f1-filtered 1H-1H TOCSY
32	2	8	2D fi1-filtered 1H-1H TOCSY
33	1	7	2D f1,f2-filtered 1H-1H NOESY
34	1	9	2D 1H-1H TOCSY
35	2	10	2D 1H-1H TOCSY
36	2	11	3D HCP
37	2	4	2D H(CC)H-TOCSY
4	1	2	2D HNN-COSY
5	1	2	2D CPMG-NOESY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.2	1	283
2	6.2	1	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	10