9HRG
Crystal structure of the Class V (G61A) GTP aptamer variant in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.215, 126.247, 68.601 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 34.600 - 2.050 |
| R-factor | 0.2081 |
| Rwork | 0.207 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.600 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.063 | |
| Rpim | 0.026 | 0.877 |
| Number of reflections | 54475 | 5416 |
| <I/σ(I)> | 13.23 | 0.84 |
| Completeness [%] | 97.8 | 97.85 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | methylpentanediol, sodium chloride, potassium chloride, magnesium chloride, spermine, cacodylate |
